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Synthesis of medium‐chain glycerides using lipase from Candida rugosa
Author(s) -
Wong W. C.,
Basri M.,
Razak C. N. A.,
Salleh A. B.
Publication year - 2000
Publication title -
journal of the american oil chemists' society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.512
H-Index - 117
eISSN - 1558-9331
pISSN - 0003-021X
DOI - 10.1007/s11746-000-0013-9
Subject(s) - candida rugosa , lipase , glyceride , capric acid , chemistry , glycerol , chromatography , triacylglycerol lipase , yield (engineering) , molar ratio , partition coefficient , organic chemistry , enzyme , fatty acid , catalysis , materials science , lauric acid , metallurgy
Enzymatic synthesis of medium‐chain glycerides (MCG) from capric acid and glycerol was studied using lipase from Candida rugosa . The effects of various reaction parameters such as time, molar ratio of substrates (mmol capric acid/mmol glycerol), amount of lipase, type of organic solvents, and initial water activity ( a w ) were studied. The best conditions tested for MCG synthesis at 37°C were, respectively, time, 24 h; molar ratio of substrates, 2.5; and amount of lipase, 100.0 mg. The use of organic solvents greatly influenced the activity of lipase in the synthesis of MCG. Generally, activity of lipase was high in nonpolar solvents with log P values from 3.50 to 4.50, where P is the partition coefficient between water and 1‐octanol. The enzymatic synthesis of MCG was preferably carried out at an initial a w of 0.328, which resulted in maximal yield. Analysis of the products of reaction using gas chromatography showed that lipase from Candida rugosa seemed to produce more dicaprin and tricaprin than monocaprin.