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A rapid and quantitative method for total fatty acid analysis of fungi and other biological samples
Author(s) -
Sönnichsen M.,
Müller B. W.
Publication year - 1999
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-999-0488-z
Subject(s) - derivatization , polyunsaturated fatty acid , chromatography , fatty acid , chemistry , gas chromatography , quantitative analysis (chemistry) , clinical chemistry , lipidology , extraction (chemistry) , methylation , biochemistry , high performance liquid chromatography , gene
A quantitative method for the one‐step esterification and determination of absolute amounts of polyunsaturated fatty acids in biotechnologically produced fungal mycelia is proposed. A system of two internal standards was used to quantify the total fatty acid content of the samples by gas chromatography. The degree of methylation of the analyzed fatty acids was calculated using the internal standards, and subsequently this quotient was used to validate the derivatization and extraction reactions. By utilizing this degree of methylation, the corrected amounts of the various fatty acids in the sample can be calculated.