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Fatty acid Δ 9 ‐Desaturation in the Triatoma infestans fat body: Response to food and trehalose administrationsin the Triatoma infestans fat body: Response to food and trehalose administrations
Author(s) -
González María S.,
Brenner Rodolfo R.
Publication year - 1999
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-999-0472-7
Subject(s) - triatoma infestans , trehalose , palmitic acid , hemolymph , nymph , stearic acid , food science , incubation , biology , fatty acid , instar , biochemistry , chemistry , botany , parasite hosting , trypanosoma cruzi , organic chemistry , world wide web , computer science , larva
The effects of food intake and carbohydrate administration on fatty acid Δ 9 ‐desaturation were investigated in isolated microsomes from Triatoma infestans fat body. Fifth instar nymphs, which were solely blood‐fed just after the molt and then fasted, were used as controls and for determination of optimal assay conditions. Both [1‐ 14 C]palmitic and [1‐ 14 C]stearic acids in a medium containing ATP, CoA, MgCl 2 , NADH, NaF, and O 2 were tested. For the control group, optimal conditions were a pH of 6.8–7.2, an incubation temperature of 29°C, and an incubation time of 10 min. Δ 9 ‐desaturation depended on the presence of reduced pyridine dinucleotides; NADH and NADPH were equally efficient. Stearic acid showed a higher apparent V max than palmitic acid, but the apparent K m were very similar. When fifth instar nymphs were blood‐fed weekly, a marked increase of Δ 9 ‐desaturation was observed for both acids. Higher desaturation activity was also induced by injection of the carbohydrate trehalose into the hemolymph of fasted nymphs. These results indicate that insect Δ 9 ‐desaturation, like the mammalian counterpart, is sensitive to dietary changes and carbohydrate administration. *** DIRECT SUPPORT *** A01K6035 00008