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Solubilization and stabilization of carotenoids using micelles: Delivery of lycopene to cells in culture
Author(s) -
Xu Xiaoying,
Wang Yan,
Constantinou Andreas I.,
StacewiczSapuntzakis Maria,
Bowen Phyllis E.,
Breemen Richard B.
Publication year - 1999
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-999-0454-9
Subject(s) - lycopene , micelle , chemistry , chromatography , lncap , cytotoxicity , solubility , solubilization , cell culture , carotenoid , biochemistry , aqueous solution , organic chemistry , in vitro , biology , prostate , genetics , cancer
The use of the organic cosolvents tetrahydrofuran and dimethylsulfoxide was found to be unsuitable for prostate tunor cell cultures because of solvent cytotoxicity and the poor solubility and instability of lycopene. For example, the half‐life of lycopene in organic/aqueous solution was found to be less than 2 h. Therefore, a micellar preparation of lycopene was developed for the solubilization and stabilization of lycopene in cell culture media. Neither the micelles themselves nor lycopene solubilized in micelles at concentrations up to 10 μg/mL. in the cell culture media produced cytotoxicity or inhibition of cell proliferation in either LNCaP human prostate cells or Hs888Lu human lung cells. Lycopene solubilized in micelles was stable for at least 96 h under standard cell culture conditions so that a constant lycopene supply could be provided to the cells. During the culture process, lycopene was taken up by LNCaP cells and reached a plateau at approximately 12 h. Micelles provide a convenient, inexpensive, and nontoxic vehicle for dissolving and stabilizing carotenes such as lycopene in tissue culture media and then delivering them to cells growing in culture.