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Induction of apoptosis and apoptotic mediators in balb/C splenic lymphocytes by dietary n−3 and n−6 fatty acids
Author(s) -
Avula C. P. Reddy,
Zaman A. K.,
Lawrence R.,
Fernandes G.
Publication year - 1999
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-999-0441-1
Subject(s) - polyunsaturated fatty acid , weanling , apoptosis , splenocyte , endocrinology , medicine , corn oil , fish oil , fas ligand , propidium iodide , biology , chemistry , spleen , biochemistry , fatty acid , programmed cell death , fishery , fish <actinopterygii>
The present study was designed to investigate the effect of diatery n−6 and n−3 polyunsaturated fatty acids (PUFA) on anti‐CD3 and anti‐Fas antibody‐induced apoptosis and its mediators in mouse spleen cells. Nutritionally adequate semipurified diets containing either 5% w/w corn oil (n−6 PUFA) or fish oil (n−3 PUFA) were fed to weanling female Balb/C mice, and 24 wk later mice were sacrificed. In n−3 PUFA‐fed mice, serum and splenocyte lipid peroxides were increased by 20 and 28.3% respectively, compared to n−6 PUFA‐fed mice. Further, serum vitamin F levels were decreased by 50% in the n−3 PUFA‐fed group, whereas higher anti0Fas‐ and anti‐CD3‐induced apoptosis (65 and 66%) and necrosis (17 and 25%), compared to the n−6 PUFA‐fed group, were found when measured with Annexin V and propidium iodide staining, respectively. In addition, decreased Bcl‐2 and increased Fas‐ligand (Fas‐L) also were observed in the n−3 PUFA‐fed group compared to the n−6 PUFA‐fed group. No difference in the ratio of splenocyte subsets nor their Fas expression was observed between the n−3 PUFA‐fed and n−6 PUFA‐fed groups, whereas decreased proliferation of splenocytes was found in n−3 PUFA‐fed mice compared to n−6 PUFA‐fed mice. In conclusion, our results indicate that dietary n−3 PUFA induces higher apoptosis by increasing the generation of lipid peroxides and elevating Fas‐L expression along with decreasing Bcl‐2 expression. A reduced proliferative response of immune cells also was observed in n−3 PUFA‐fed mice.