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Cholesterol synthesis and degradation in normal rats fed a cholesterol‐free diet with excess cystine
Author(s) -
Aoyama Yoritaka,
Amano Nobuyuki,
Yoshida Akira
Publication year - 1999
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-999-0402-8
Subject(s) - cholesterol , medicine , cystine , endocrinology , cholic acid , clinical chemistry , sterol , lipidology , excretion , bile acid , chemistry , stimulation , chenodeoxycholic acid , biology , biochemistry , enzyme , cysteine
Feeding a diet with excess cystine to rats resulted in hypercholesterolemia. To understand the mechanism of the hypercholesterolemia’ cholesterol synthesis and degradation’ bile acid content of bile’ and fecal steroids were determined. The in vivo incorporation of tritiated water into hepatic cholesterol’ and activity of hepatic 3‐hydroxy‐3‐methylglutaryl‐CoA reductase in rats fed a high‐cystine diet were significantly higher than those in rats fed a control diet. The activity of hepatic cholesterol 7α‐hydroxylase was similar between two groups. Little effect of cystine supplementation was found on fecal sterol excretion although there were some changes in biliary excretion of cholic acid derivatives. These results indicate that hypercholesterolemia caused by feeding of a high‐cystine diet may be due to the stimulation of hepatic cholesterol synthesis.