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Elevated plasma levels of F 2α isoprostane in cystic fibrosis
Author(s) -
Collins Clare E.,
Quaggiotto Paul,
Wood Lisa,
O'Loughlin Edward V.,
Henty Richard L.,
Garg Manohar L.
Publication year - 1999
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-999-0397-1
Subject(s) - medicine , endocrinology , isoprostane , lipidology , chemistry , cystic fibrosis , oxidative stress , clinical chemistry , vitamin e , isoprostanes , lipid peroxidation , inflammation , antioxidant , biochemistry
Cystic fibrosis (CT) is associated with chronic lung infection’ inflammation’ and elevated indices of oxidative stress. Recently’ isoprostanes were shown to be a reliable in vivo marker of oxidant injury with 8‐ iso ‐PGF 2α ’ shown to cause airflow obstruction and plasma exudation in guinea pig lung. The present study was designed to examine the relationship between 8‐iso‐PGF 2α levels’ plasma antioxidants’ and clinical status in CF. We hypothesized that plasma 8‐iso‐PGF 2α levels would be higher in subjects with CT compared to healthy controls. Plasma 8‐iso‐PGF 2α levels were prospectively measured in 22 subjects with CF and nine healthy controls using an 8‐isoprostane enzyme immunoassay kit along with plasma vitamins A’ E’ and β‐carotene. Plasma 8‐ iso ‐PGF 2α levels were shown to be significantly elevated in the CE subjects compared to controls (319.6±52.6 vs. 145.0±21.0 pg/ml’ P =0.005). Plasma levels of antioxidants were significantly lower for the CF subjects compared to the controls (vitamin A’ P <0.003; vitamin E’ P <0.001; and β‐carotene’ P <0.01). This study confirns significantly elevated lipid peroxidation in CF using 8‐ iso ‐PGF 2α levels.