Premium
Vitamin E reduces cholesterol esterification and uptake of acetylated low density lipoprotein in macrophages
Author(s) -
Shige Hideki,
Ishikawa Toshitsugu,
Suzukawa Michio,
Nishiwaki Masato,
Yamashita Takeshi,
Nakajima Kei,
Ito Toshimitsu,
Higashi Kenji,
Ayaori Makoto,
Yonemura Atsushi,
Nestel Paul,
Nakamura Haruo
Publication year - 1998
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-998-0320-9
Subject(s) - cholesteryl ester , chemistry , cholesterol , clinical chemistry , vitamin , sterol o acyltransferase , low density lipoprotein , lipidology , medicine , endocrinology , lipoprotein , biochemistry , metabolism , acetylation , hydrolysis , biology , gene
The effects of vitamin E on cholesteryl ester (CE) metabolism in 1774 cells were examined. Pretreatment of 1774 cells with vitamin E at concentrations above 50 μM significantly decreased acetylated low density lipoprotein (LDL)‐induced incorporation of [ 14 C]oleate into CF in cells in a dose‐dependent manner. This was partly due to vitamin E Also significantly inhibiting the uptake of [ 3 H]CE‐labeled acetylated LDL by 1774 cells. A trend existed toward suppression of acyl‐CoA:cholesterol acyltransferase (ACAT) activity in the cell lysate at high vitamin E concentration, but there was no effect on hydrolysis of CE. These data indicate that vitamin E reduces the uptake of modified LDL and suppresses ACAT activity, resulting in less cholesterol esterification in macrophages; a novel mechanism underlying the antiatherogenic properties of vitamin E.