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Analysis of ubiquinone and tocopherol levels in normal and hyperlipidemic human plasma
Author(s) -
Zhang Yiyi,
Eriksson Mats,
Dallner Gustav,
Appelkvist EevaLiisa
Publication year - 1998
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-998-0274-y
Subject(s) - chemistry , chromatography , ubiquinol , extraction (chemistry) , antioxidant , tocopherol , high performance liquid chromatography , sodium borohydride , quantitative analysis (chemistry) , biochemistry , vitamin e , coenzyme q – cytochrome c reductase , cytochrome c , mitochondrion , catalysis
The type and amount of lipophilic antioxidants in plasma of hyperlipidemic patients are of great importance since they play a central role in preventing deleterious oxidation of blood lipids and proteins. Isolation and quantitation of lipophilic antioxidants from hyperlipidemic plasma samples meet great obstacles because of increased levels of various intermediary lipid products. This study was designed to develop a rapid and efficient extraction and separation procedure for simultaneous analysis of ubiquinone‐9 and‐10 as well as α‐, δ‐, and γ‐tocopherol isomers. The levels of ubiquinone‐10, α‐ and γ‐tocopherol were analyzed in human plasma samples using high‐performance liquid chromatography. Lipid extraction was performed by petroleum ether/methanol/water. After phase separation, ubiquinone was reduced to ubiquinol by sodium borohydride and the lipids were separated on a C18 column. A binary gradient with solvents containing lithium perchlorate was used, and an electrochemical detector was employed for quantitation. This procedure was also efficient for the analysis of antioxidant lipids in samples containing a large number of accumulated and interfering lipid intermediates. Thus, the procedure described here is useful for efficient and rapid quantitation of ubiquinones and tocopherols in human plasma samples, especially those originating from hyperlipidemic patients.