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Production of antiserum for sensitive enzyme‐linked immunosorbent assay of 3‐carboxy‐4‐methyl‐5‐propyl‐2‐furanpropanoic acid by chemiluminescence
Author(s) -
Tanaka Touichi,
Ikebuchi Hideharu,
Sawada Junichi,
Tanaka Yoshiko
Publication year - 1998
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-998-0264-0
Subject(s) - antiserum , chemiluminescence , conjugate , hapten , chemistry , chromatography , moiety , immunoassay , antigen , antibody , stereochemistry , biology , immunology , mathematical analysis , mathematics
Abstract To obtain a specific antiserum for use in enzymelinked immunosorbent assay (ELISA) of 3‐carboxy‐4‐methyl‐5‐propyl‐2‐furanpropanoic acid (CMPF), we prepared a haptencarrier conjugate in which the CMPF hapten was linked to a carrier protein through the 5‐(1‐hydrazopropyl) group. The antisera raised against this antigen in guinea pigs had excellent specificity for CMPF, showing little cross‐reactivity with closely related compounds and no significant cross‐reactivities with other furan compounds. The results indicated that a specific antiserum to CMPF could be produced by an antigen whose CMPF moiety is linked to a carrier protein through a position remote from the inherent functional groups. A standard curve of CMPF by ELISA using a chemiluminescence system showed a high sensitivity and a linearity in the range of 5–100 ng/mL.