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Gas chromatography‐mass spectrometry methods for structural analysis of fatty acids
Author(s) -
Christie William W.
Publication year - 1998
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-998-0214-x
Subject(s) - chemistry , derivatization , chromatography , mass spectrometry , gas chromatography , adduct , acetonitrile , gas chromatography–mass spectrometry , organic chemistry
Procedures for structural analysis of fatty acids are reviewed. The emphasis is on methods that involve gas chromatography‐mass spectrometry and, in particular, the use of picolinyl ester and dimethyloxazoline derivatives. These should be considered as complementing each other, not simply as alternatives. However, additional derivatization procedures can be of value, including hydrogenation and deuteration, and preparation of dimethyl disulfide and 4‐methyl‐1,2,4‐triazoline‐3,5‐dione adducts. Sometimes complex mixtures must be separated into simpler fractions prior to analysis by gas chromatography‐mass spectrometry. Silver ion and reversed‐phase high‐performance liquid chromatography are then of special value. In particular, a novel application of the latter technique, involving a base‐deactivated stationary phase and acetonitrile as mobile phase, is described that is suited to the separation of fatty acids in the form of picolinyl ester and dimethyloxazoline derivatives, as well as methyl esters.