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Fatty acid composition of the cellular slime mold Polysphondylium pallidum
Author(s) -
Saito Tamao,
Ochiai Hiroshi
Publication year - 1998
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-998-0212-z
Subject(s) - cyclopropane , slime mold , dictyostelium discoideum , fatty acid , biochemistry , biology , bacteria , chemistry , organic chemistry , ring (chemistry) , genetics , gene
The cellular slime mold Polysphondylium pallidum was grown upon Escherichia coli B/r, and the fatty acid compositions of total lipids obtained from vegetative amebae and aggregation‐competent cells were compared. Fatty acids isolated from vegetative cells included C‐17 and C‐19 cyclopropane fatty acids and also straight‐chain, saturated fatty acids. The cyclopropane fatty acids were derived from the ingested bacteria. Development of amebae to aggregation‐competent cells was accompanied by a substantial decrease in saturated cyclopropane fatty acids and a concomitant increase in unsaturated fatty acids and unsaturated cyclopropane fatty acids, mostly as 18∶3 (5,9,12). We report here the fatty acid composition and identify the occurrence of Δ5 desaturation of cyclopropane fatty acids, namely, 9,10‐methylene 5‐hexadecenoic acid and 11,12‐methylene 5‐octadecenoic acid. These fatty acids have not been reported previously in the related species Dictyostelium discoideum , which also feeds on E. coli B/r and has Δ5‐desaturation activity.