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Enhancement of low density lipoprotein binding to both low density lipoprotein receptor‐positive and‐ negative cells by tetracycline antibiotics
Author(s) -
Miura Shinji,
Hasumi Keiji,
Takayasu Ritsuko,
Sugimoto Maki,
Endo Akira
Publication year - 1998
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-998-0177-y
Subject(s) - lipidology , clinical chemistry , tetracycline , low density lipoprotein , lipoprotein , chemistry , antibiotics , receptor , ldl receptor , microbiology and biotechnology , biochemistry , cholesterol , biology
Some tetracycline (TC) antibiotics, including TC and anhydrotetracycline, have been found to enhance specific binding of low density lipoprotein (LDL) to both LDL receptor‐positive and‐negative cells at relatively higher concentrations. When incubated at 37°C, the ability of LDL receptor‐negative human fibroblasts to bind 125 I‐LDL was increased from<2 to 45 ng/mg by 170 μM TC. In normal human fibroblasts and Hep G2 cells, 125 I‐LDL binding was elevated 1.4‐ to 2‐fold by 113 μM TC. The 125 I‐LDL binding in the presence of TC was diminished by both heparin and EDTA. The enhancement by TC was not observed when 125 I‐LDL binding was assayed at 4°C. TC enhanced LDL binding to paraformaldehyde‐fixed Hep G2 cells, excluding LDL receptor induction in the mechanism. These results demonstrated that TC enhanced cellular LDL binding through a process not involving functional LDL receptors.