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Lipids of hamster cheek pouch epithelium
Author(s) -
Whittle Sara,
Swartzendruber Donald C.,
Kremer Mary,
Squier Christopher A.,
Wertz Philip W.
Publication year - 1997
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-997-0124-y
Subject(s) - cheek pouch , stratum corneum , corneocyte , hamster , lipidology , biochemistry , biology , clinical chemistry , phospholipid , epithelium , barrier function , chemistry , microbiology and biotechnology , membrane , genetics
The hamster cheek pouch is a much used but incompletely understood experimental model. In particular, the cheek pouch epithelial lipids, which are important for permeability barrier function as well as other aspects of epithelial biology, have not been completely characterized. In the present study, the complete lipid class composition has been determined by thin‐layer chromatography in conjunction with photodensitometry. The major lipid classes were phospholipids, free sterols, and ceramides. Minor amounts of monohexosylceramides, sterol esters, fatty acids, and triglycerides were also present. Significant amounts of covalently bound ω‐hydroxyceramide was also detected. Transmission electron micrographs reveal extensive, largely paired, lipid bilayers in the intercellular spaces of the stratum corneum.