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Effects of oxidative stress on glycerolipid acyl turnover in rat hepatocytes
Author(s) -
GirónCalle Julio,
Schmid Patricia C.,
Schmid Harald H. O.
Publication year - 1997
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-997-0118-9
Subject(s) - phosphatidylethanolamine , polyunsaturated fatty acid , phosphatidylcholine , biochemistry , clinical chemistry , lipidology , fatty acid , lipid peroxidation , chemistry , oxidative stress , thiobarbituric acid , incubation , phospholipid , membrane
Lipid peroxidation was induced in freshly isolated rat hepatocytes by incubation in the presence of Fe 3+ , resulting in accumulation of thiobarbituric acid reactive substances. Analysis of lipid classes revealed that the levels and fatty acid compositions of the two major phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE), remained unchanged but the levels of triacylglycerols (TAG) were significantly reduced, and some of their polyunsaturated fatty acids were selectively lost as the result of oxidant treatment. Acyl turnover in PC and PE as determined by 18 O incorporation from H 2 18 O‐containing media remained largely unchanged during oxidant treatment, while some increased turnover of the saturated fatty acids in TAG was observed. We hypothesize that constitutive recycling of membrane phospholipids rather than selective in situ repair eliminates peroxidized species of PC and PE, TAG could serve as an expendable fatty acid reserve, providing a limited but very dynamic pool of polyunsaturated fatty acids for the resynthesis of phospholipids.