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Thermotropic phase behavior of in vivo extracted human stratum corneum lipids
Author(s) -
Bonté Frédéric,
Pinguet Patrick,
Saunois Alex,
Meybeck Alain,
Beugin Sophie,
Ollivon Michel,
Lesieur Sylviane
Publication year - 1997
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-997-0083-3
Subject(s) - stratum corneum , differential scanning calorimetry , thermotropic crystal , chemistry , corneocyte , chromatography , ceramide , calorimetry , biophysics , phase (matter) , biochemistry , organic chemistry , biology , liquid crystalline , thermodynamics , apoptosis , genetics , physics
The thermotropic phase behavior of lipids extracted either in vivo from inner forearm (SCLE) or plantar callus (PC) was investigated by differential scanning calorimetry and small angle X‐ray diffraction. PC composition was chromatographically modified (MPC) by eliminating the more polar lipids in order to evaluate their role. Analysis of composition confirms the potential use of PC as a source of stratum corneum lipids. MPC and SCLE exhibit similar differential scanning calorimetry (DSC) profiles with a main transition around 50°C attributed to the solid‐to‐liquid phase transition of the ceramides. The absence of a transition around 50°C for PC suggests the possible perturbation of ceramide packing by the significantly high proportion of phospholipids. X‐ray data suggest a high miscibility of sebum components in stratum corneum lipids with possible modification of chain packing. The MPC patterns show a lipid phase separation which underscores the role of polar lipids in cholesterol/free fatty acids/sterol esters/ceramides structural cohesion.