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Expression of fatty acyl‐CoA binding proteins in colon cells: response to butyrate and transformation
Author(s) -
Gossett Ruanna E.,
Schroeder Friedhelm,
Gunn J. Martyn,
Kier Ann B.
Publication year - 1997
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-997-0073-5
Subject(s) - butyrate , fatty acid binding protein , cytosol , cell culture , biochemistry , cell , biology , binding protein , adipocyte protein 2 , chemistry , microbiology and biotechnology , fatty acid , gene , genetics , fermentation , enzyme
Fatty acyl‐CoA affect many cellular functions as well as serving as cellular building blocks. Several families of cytosolic fatty acyl‐CoA binding proteins may modulate the activities of fatty acyl‐CoA. Intestinal enterocytes contain at least three unique families of cytosolic proteins that bind fatty acyl‐CoA: acyl‐CoA binding protein (ACBP), fatty acid binding proteins (including the liver, L‐FABP and intestinal, I‐FABP), and sterol carrier protein‐2 (SCP‐2). Immortalized rat colon epithelial cell lines expressed only ACBP and SCP‐2 at levels of 0.75±0.13 and 0.42±0.02 ng/μg protein. Ras and src transformation increased colon cell density and differentially altered ACBP and SCP‐2 expression without affecting I‐FABP or L‐FABP levels. ACBP levels were 1.8‐fold and 1.5‐fold increased in ras ‐ and src ‐transformed cells, respectively. In contrast, SCP‐2 expression was significantly decreased 55 and 67% in ras ‐ and src ‐transformed cells, respectively. Butyrate treatment of ras ‐ and src ‐transformed cells decreased cell proliferation up to 60–85% as compared to 25–30% in control cells. Butyrate treatment decreased ACBP expression in all cell lines but had no effect on the levels of SCP‐2, I‐FABP, or L‐FABP. These studies suggest that the differential expression of ACBP and SCP‐2 in rat colonic cell lines, as well as their modulation by butyrate, may be altered by cell transformation.