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Analysis of lipoxygenase‐derived fatty acid hydroperoxides by electrospray ionization tandem mass spectrometry
Author(s) -
Schneider Claus,
Schreier Peter,
Herderich Markus
Publication year - 1997
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-997-0041-0
Subject(s) - chemistry , tandem mass spectrometry , derivatization , electrospray ionization , mass spectrometry , extractive electrospray ionization , chromatography , protein mass spectrometry , electrospray , collision induced dissociation , chemical ionization , sample preparation in mass spectrometry , direct electron ionization liquid chromatography–mass spectrometry interface , ionization , organic chemistry , ion
A rapid method is described for the identification of fatty acid hydroperoxides by electrospray ionization‐tandem mass spectrometry coupled to liquid chromatography without any derivatization required prior to analysis. Localization of fatty acid hydroperoxides in complex mixtures was achieved by monitoring the loss of hydrogen peroxide using constant neutral loss scanning. In the presence of 5 mM NH 4 OAc in methanol‐water, adductions [M+NH 4 ] + were formed almost exclusively, directly revealing the molecular mass of the thermolabile hydroperoxides. In addition, low energy collision‐induced dissociation of precursor ions [M+NH 4 ] + led to characteristic product ions from both the 9‐ and 13‐regioisomers. Thus, electrospray ionization‐tandem mass spectrometry provides a straightforward approach for the study of the regioselectivity of lipoxygenase catalysis without any derivatization step required prior to analysis.