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Biotransformations of tocopherols by Streptomyces catenulae
Author(s) -
Rousseau Brigitte,
Dostal Larry,
Rosazza John P. N.
Publication year - 1997
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-997-0012-5
Subject(s) - nitration , chemistry , tocopherol , streptomyces , mass spectrometry , nitrite , nitrate , organic chemistry , antioxidant , chromatography , bacteria , vitamin e , biology , genetics
Streptomyces catenulae catalyzed the oxidation of α‐tocopherol to α‐tocopherolquinone. Nitrotocopherols isolated from S. catenulae grown in defined culture medium containing δ‐and γ‐tocopherols are formed by a combination of enzymatic nitrate reduction to nitrite, and subsequent nonenzymatic acid‐catalyzed nitration. The incorporation of 15 N 18 O 3 ‐into nitrated tocopherols confirmed the origin of the nitrating species. Structures of chromatographically purified products obtained from S. catenulae transformations of tocopherols were deduced by spectral (mass spectrometry, 1 H‐, and 13 C‐nuclear magnetic resonance) analyses.