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Correlation of ATP/citrate lyase activity with lipid accumulation in developing seeds of Brassica napus L.
Author(s) -
Ratledge Colin,
Bowater Moray D. V.,
Taylor Philip N.
Publication year - 1997
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-997-0002-7
Subject(s) - atp citrate lyase , brassica , biochemistry , organelle , chloroplast , biosynthesis , acetyl coa carboxylase , sinapis , differential centrifugation , biology , sucrose , enzyme , lyase , chemistry , pyruvate carboxylase , botany , citrate synthase , gene
The temporal distribution of ATP/citrate lyase (ACL) activity in developing seeds of Brassica napus L. closely paralleled both that of acetyl‐CoA carboxylase and the overall rate of lipid biosynthesis. Maximum ACL activities (250 nmol acetyl‐CoA formed min −1 ·g fresh seed) were recorded between 35 to 42 d after pollination and, if the in vitro data could be extrapolated to the situation in vivo , could account for half of the acetyl‐CoA required for the measured rate of fatty acid biosynthesis during seed development. The enzyme appeared to be localized in a subcellular compartment, which was clearly separated from mitochondria on a sucrose gradient and by differential centrifugation, and which corresponded to the chloroplast organelle.