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Pathways for fatty acid elongation and desaturation in Neurospora crassa
Author(s) -
McKeon Thomas A.,
GoodrichTanrikulu Marta,
Lin JiannTsyh,
Stafford Allan
Publication year - 1997
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-997-0001-8
Subject(s) - neurospora crassa , fatty acid , lipidology , biochemistry , phospholipid , neurospora , chemistry , clinical chemistry , elongation , biology , gene , membrane , mutant , materials science , ultimate tensile strength , metallurgy
Neurospora crassa incorporated exogenous deuterated palmitate (16∶0) and 14 C‐labeled oleate (18∶1 Δ9 ) into cell lipids. Of the exogenous 18∶1 Δ9 incorporated, 59% was desaturated to 18∶2 Δ9,12 and 18∶3 Δ9,12,15 . Of the exogenous 16∶0 incorporated, 20% was elongated to 18∶0, while 37% was elongated and desaturated into 18∶1 Δ9 , 18∶2 Δ9,12 , and 18∶3 Δ9,12,15 . The mass of unsaturated fatty acids in phospholipid and triacylglycerol is 12 times greater than the mass of 18∶0. Deuterium label incorporation in unsaturated fatty acids is only twofold greater than in 18∶0, indicating a sixfold preferential use of 16∶0 for saturated fatty acid synthesis. These results indicate that the release of 16∶0 from fatty acid synthase is a key control point that influences fatty acid composition in Neurospora .
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