Premium
Effect of Fabp1/Scp ‐ 2/Scp ‐ x Ablation on Whole Body and Hepatic Phenotype of Phytol‐Fed Male Mice
Author(s) -
Landrock Danilo,
Milligan Sherrelle,
Martin Gregory G.,
McIntosh Avery L.,
Landrock Kerstin K.,
Schroeder Friedhelm,
Kier Ann B.
Publication year - 2017
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-017-4249-y
Subject(s) - lipidology , clinical chemistry , phytol , phenotype , biology , endocrinology , medicine , chemistry , gene , biochemistry
Liver fatty acid binding protein ( Fabp1 ) and sterol carrier protein‐2/sterol carrier protein‐x ( SCP ‐ 2/SCP ‐ x ) genes encode proteins that enhance hepatic uptake, cytosolic transport, and peroxisomal oxidation of toxic branched‐chain fatty acids derived from dietary phytol. Since male wild‐type (WT) mice express markedly higher levels of these proteins than females, the impact of ablating both genes (TKO) was examined in phytol‐fed males. In WT males, high phytol diet alone had little impact on whole body weight and did not alter the proportion of lean tissue mass (LTM) versus fat tissue mass (FTM). TKO conferred on dietary phytol the ability to induce weight loss as well as reduce liver weight, FTM, and even more so LTM. Concomitantly TKO induced hepatic lipid accumulation, preferentially threefold increased phospholipid (PL) at the expense of decreased triacylglycerol (TG) and total cholesterol. Increased PL was associated with upregulation of membrane fatty acid transport/translocase proteins (FATP 2,4), cytosolic fatty acid/fatty acyl‐CoA binding proteins (FABP2, ACBP), and the rate limiting enzyme in PL synthesis ( Gpam ). Decreased TG and cholesterol levels were not attributable to altered levels in respective synthetic enzymes or nuclear receptors. These data suggest that the higher level of Fabp1 and Scp2/Scpx gene products in WT males was protective against deleterious effects of dietary phytol, but TKO significantly exacerbated phytol effects in males.