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Non‐conjugated cis / trans 18:2 in Beef Fat are Mainly Δ‐9 Desaturation Products of trans ‐18:1 Isomers
Author(s) -
Vahmani P.,
Rolland D. C.,
Gzyl K. E.,
Dugan M. E. R.
Publication year - 2016
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-016-4207-0
Subject(s) - chemistry , cis–trans isomerism , adipose tissue , ruminant , conjugated linoleic acid , fatty acid , food science , stereochemistry , biology , biochemistry , linoleic acid , ecology , crop
Human liver cells (HepG2) were cultured with individual trans ( t ) 18:1 including t 6‐, t 12‐, t 13‐, t 14‐, t 15‐ and t 16‐18:1, and retention times of their Δ‐9 desaturation products were determined using 100‐m biscyanopropyl‐polysiloxane and SLB‐IL111 columns. Corresponding peaks were found in beef adipose tissues known to have different delta‐9 desaturase activities. Further lines of evidence indicating the presence of Δ‐9 desaturation products of t ‐18:1 isomers in beef fat were developed by analysis of fatty acid methyl esters (FAME) fractionated using Ag + ‐TLC, and by GC/MS. Some of the Δ‐9 desaturation products of t ‐18:1 have been previously identified in ruminant fat ( c 9, t 12‐ and c 9, t 13‐18:2). Some of the Δ‐9 desaturation products of t ‐18:1 ( c 9, t 14‐ and c 9, t 15‐18:2) have been previously tentatively identified as different fatty acids, and for the first time we provide evidence of the presence of c 9, t 16‐18:2, and where t 6, c 9‐18:2 may elute during analysis of FAME from beef fat.