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Danshensu Promotes Cholesterol Efflux in RAW264.7 Macrophages
Author(s) -
Gao Hui,
Li Lingyan,
Li Lan,
Gong Bo,
Dong Pengzhi,
Fordjour Patrick Asare,
Zhu Yan,
Fan Guanwei
Publication year - 2016
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-016-4178-1
Subject(s) - abca1 , abcg1 , cd36 , scavenger receptor , foam cell , cholesterol , efflux , simvastatin , intracellular , chemistry , downregulation and upregulation , thp1 cell line , lipoprotein , microbiology and biotechnology , biochemistry , biology , pharmacology , cell culture , receptor , transporter , gene , genetics
Contemporary research suggests that macrophage foam cell and cholesterol efflux defect play pivotal role in atherogenesis. We reported on the heretofore unknown therapeutic effect of Danshensu (DSS) in reducing intracellular cholesterol level and unraveled the mechanism of DSS promotes cholesterol efflux. Oxidized low‐density lipoprotein stimulation of Raw264.7 cells into foam cells, which were treated with DSS and co‐treated with Simvastatin and Rosiglitazone. PPARγ, ABCA1, ABCG1, SR‐BI, CD36, and LXR‐α mRNA were quantified by Real‐Time PCR. Western blotting was used to determine protein expression of PPARγ, ABCA1 and CD36. Cellular cholesterol handling was studied by measurement of intracellular lipid droplets concentration and cholesterol efflux. DSS significantly reduced scavenger receptor CD36 and its orthologue SR‐BI. In addition, DSS stimulated the upregulation of cellular cholesterol exporters ABCA1 and ABCG1 to reduce intracellular lipid accumulation. DSS can reduce lipid deposition in Raw264.7 foam cells by balancing CD36 and ABCA1 protein expression.