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Caloric Restriction Normalizes Obesity‐Induced Alterations on Regulators of Skeletal Muscle Growth Signaling
Author(s) -
Dungan Cory M.,
Li Ji,
Williamson David L.
Publication year - 2016
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-016-4168-3
Subject(s) - caloric theory , endocrinology , skeletal muscle , medicine , obesity , clinical chemistry , mtorc1 , lipidology , biology , pi3k/akt/mtor pathway , chemistry , signal transduction , microbiology and biotechnology
The objective of this study was to establish the impact of caloric restriction on high fat diet‐induced alterations on regulators of skeletal muscle growth. We hypothesized that caloric restriction would reverse the negative effects of high fat diet‐induced obesity on REDD1 and mTOR‐related signaling. Following an initial 8 week period of HF diet‐induced obesity, caloric restriction (CR ~30 %) was employed while mice continued to consume either a low (LF) or high fat (HF) diet for 8 weeks. Western analysis of skeletal muscle showed that CR reduced ( p < 0.05) the obesity‐related effects on the lipogenic protein, SREBP1. Likewise, CR reduced ( p < 0.05) the obesity‐related effects on the hyperactivation of mTORC1 and ERK1/2 signaling to levels comparable to the LF mice. CR also reduced ( p < 0.05) obesity‐induced expression of negative regulators of growth, REDD1 and cleaved caspase 3. These findings have implications for on the reversibility of dysregulated growth signaling in obese skeletal muscle, using short‐term caloric restriction.