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Myristic Acid Enhances Diacylglycerol Kinase δ‐Dependent Glucose Uptake in Myotubes
Author(s) -
Wada Yuko,
Sakiyama Shizuka,
Sakai Hiromichi,
Sakane Fumio
Publication year - 2016
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-016-4162-9
Subject(s) - myristic acid , diacylglycerol kinase , palmitic acid , glucose uptake , snf3 , myogenesis , c2c12 , biochemistry , chemistry , stearic acid , protein kinase c , medicine , biology , endocrinology , kinase , insulin , fatty acid , in vitro , organic chemistry
Decreased expression of diacylglycerol kinase (DGK) δ in skeletal muscles attenuates glucose uptake and is closely related to the pathogenesis of type 2 diabetes. Therefore, up‐regulation of DGKδ expression is thought to protect and improve glucose homoeostasis in type 2 diabetes. We recently determined that myristic acid (14:0), but not palmitic (16:0) or stearic (18:0) acid, significantly increased DGKδ2 protein expression in mouse C2C12 myotubes. In the current study, we analyzed whether myristic acid indeed enhances glucose uptake in C2C12 myotubes. We observed that myristic acid caused ~1.4‐fold increase in insulin‐independent glucose uptake. However, palmitic and stearic acids failed to enhance glucose uptake. DGKδ‐specific siRNA decreased myristic acid‐dependent increase of glucose uptake. Moreover, overexpression of DGKδ2 enhanced glucose uptake in C2C12 cells in the absence of myristic acid treatment. Taken together, these results strongly suggest that myristic acid enhances basal glucose uptake in myotubes in a DGKδ2 expression‐dependent manner.