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Dietary Black Raspberry Seed Oil Ameliorates Inflammatory Activities in db/db Mice
Author(s) -
Lee Hee Jae,
Jung Hana,
Cho Hyunnho,
Lee Kiuk,
Kwak HoKyung,
Hwang Keum Taek
Publication year - 2016
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-016-4159-4
Subject(s) - adipose tissue , chemistry , leptin , endocrinology , medicine , biology , biochemistry , obesity
Abstract The objective of this study was to evaluate the status of the markers related to inflammation in db/db mice fed black raspberry seed (BRS) oil, which is rich in α‐linolenic acid. Mice were divided into four groups: (1) C57BL/6 mice fed 16 % calories from soybean oil (normal CON); (2) C57BL/KsJ‐ db/db mice fed 16 % calories from soybean oil (CON); (3) C57BL/KsJ‐ db/db mice fed 8 % calories from soybean and 8 % calories from BRS oil (BRS 50 %); and (4) C57BL/KsJ‐ db/db mice fed 16 % calories from BRS oil (BRS 100 %). After 10 weeks, n‐6/n‐3 fatty acid ratios were significantly ( P < 0.05) lower in the livers and epididymal adipose tissues of the BRS 50 % and BRS 100 % mice than in the CON. Serum TNFα and IL‐6 were significantly ( P < 0.05) lower in the BRS 50 % and BRS 100 % than in the CON. Serum IL‐10 was significantly ( P < 0.05) higher in the BRS 100 % than the CON. In the liver and epididymal adipose tissue, mRNA levels of pro‐inflammatory markers in the BRS 50 % and BRS 100 % were lower than in the CON. Anti‐inflammatory markers were higher in the epididymal adipose tissues of the BRS 50 % and BRS 100 % than in the CON. In the epididymal adipose tissue, macrophage infiltration markers (F4/80 and CD68) and leptin mRNA were significantly ( P < 0.05) lower in the BRS 50 % and BRS 100 % than in the CON. Results of this study suggest that BRS oil may have anti‐inflammatory effects in obese diabetic mice by ameliorating inflammatory responses.