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4‐Hydroxyderricin, as a PPARγ Agonist, Promotes Adipogenesis, Adiponectin Secretion, and Glucose Uptake in 3T3‐L1 Cells
Author(s) -
Li Yongjia,
Goto Tsuyoshi,
Yamakuni Kanae,
Takahashi Haruya,
Takahashi Nobuyuki,
Jheng HueiFen,
Nomura Wataru,
Taniguchi Masahiko,
Baba Kimiye,
Murakami Shigeru,
Kawada Teruo
Publication year - 2016
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-016-4154-9
Subject(s) - adipogenesis , 3t3 l1 , endocrinology , medicine , adiponectin , adipocyte , insulin resistance , glucose uptake , glucose transporter , peroxisome proliferator activated receptor , downregulation and upregulation , chemistry , insulin , protein kinase b , biology , receptor , adipose tissue , phosphorylation , biochemistry , gene
Adipocyte differentiation plays a pivotal role in maintaining the production of small‐size adipocytes with insulin sensitivity, and impaired adipogenesis is implicated in insulin resistance. 4‐Hydroxyderricin (4‐HD), a phytochemical component of Angelica keiskei , possesses diverse biological properties such as anti‐inflammatory, antidiabetic, and antitumor. In the present study, we investigated the effects of 4‐HD on adipocyte differentiation. 4‐HD promoted lipid accumulation in 3T3‐L1 cells, upregulated both peroxisome proliferator‐activated receptor (PPAR)‐γ mRNA and protein expression, and acted as a ligand for PPARγ in the luciferase assay. Moreover, 4‐HD increased the mRNA and protein expression levels of adiponectin. Additionally, it promoted insulin‐dependent glucose uptake into 3T3‐L1 adipocytes and increased Akt phosphorylation and glucose transporter (GLUT) 4 mRNA expression. In summary, these findings suggest that 4‐HD, which promoted adipogenesis and insulin sensitivity in 3T3‐L1 cells, might be a phytochemical with potent insulin‐sensitizing effects.