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Structural Characterization of Neutral Glycosphingolipids from 3T3‐L1 Adipocytes
Author(s) -
Kojima Hisao,
Suzuki Yusuke,
Ito Masahiro,
Kabayama Kazuya
Publication year - 2015
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-015-4035-7
Subject(s) - glycosphingolipid , adipocyte , lipidology , adipose tissue , chemistry , insulin resistance , lipidomics , clinical chemistry , 3t3 l1 , biochemistry , pathogenesis , sphingolipid , medicine , endocrinology , biology , obesity
In recent years, obesity has been considered a pathological stage of early lifestyle‐related diseases, and adipose tissue and adipocyte research has been active. Glycosphingolipids are involved in the pathogenesis of type 2 diabetes induced by insulin resistance, but the details of the glycosphingolipid molecular species composition of adipocytes have yet to be elucidated. We used 3T3‐L1 adipocytes and the 1,2‐dichloroethane‐wash method to remove triacylglycerols, which are abundant in adipocytes, and analyzed the structures of glycosphingolipids, particularly neutral glycosphingolipids, using liquid chromatography–mass spectrometry.