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miRNA Expression in Human Intestinal Caco‐2 Cells is Comparably Regulated by cis ‐ and trans ‐Fatty Acids
Author(s) -
Köpke Solveigh,
Buhrke Thorsten,
Lampen Alfonso
Publication year - 2015
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-015-3988-x
Subject(s) - downregulation and upregulation , microrna , fatty acid , apoptosis , fatty acid synthesis , gene expression , chemistry , biology , biochemistry , gene , microbiology and biotechnology
Trans ‐fatty acids are unsaturated fatty acids with at least one double bond in trans configuration. While their role in the development of coronary heart disease is broadly accepted, a potential impact of these fatty acids on colon carcinogenesis is still under discussion. MiRNAs are small non‐coding RNAs that regulate the gene expression at a post‐transcriptional level by inhibiting the translation of target mRNAs. We investigated the effect of 16 different C 18 fatty acid isomers on the expression of 84 cancer‐related miRNAs in the human colorectal adenocarcinoma cell line Caco‐2 by using a qRT‐PCR array. 66 of these 84 miRNAs were deregulated by at least one fatty acid, however, there was no trans ‐specific impact on miRNA expression as the corresponding cis isomer of a given fatty acid generally had comparable effects on the miRNA expression profile. The most pronounced effects were observed for hsa‐miR‐146a‐5p, which was upregulated by four of the 16 investigated fatty acids, and hsa‐miR‐32‐5p, which was strongly downregulated by five fatty acids. As hsa‐miR‐32‐5p was described to target genes being involved in the regulation of apoptosis, the effect of α‐eleostearic acid on the expression of the apoptosis‐associated genes BCL2L11, BCL‐2, and BCL‐XL was examined. The qPCR results indicate that fatty acid‐mediated downregulation of hsa‐miR‐32‐5p is accompanied by a downregulation of BCL‐2 and BCL2L11 mRNA whereas BCL‐XL was shown to be simultaneously upregulated. In conclusion, our data indicate that several fatty acids are able to regulate miRNA expression of human colon cancer cells. However, no trans ‐specific regulation was observed.