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Rosiglitazone, a PPAR‐γ Agonist, Fails to Attenuate CLA‐Induced Milk Fat Depression and Hepatic Lipid Accumulation in Lactating Mice
Author(s) -
Vyas Diwakar,
Teter Beverly B.,
Delmonte Pierluigi,
Erdman Richard A.
Publication year - 2014
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-014-3906-7
Subject(s) - endocrinology , medicine , conjugated linoleic acid , lipogenesis , fatty acid synthase , lactation , steatosis , fatty liver , chemistry , fatty acid synthesis , sterol regulatory element binding protein , fatty acid , biology , linoleic acid , lipid metabolism , cholesterol , biochemistry , sterol , pregnancy , genetics , disease
Our objective was to investigate the combination of rosiglitazone (ROSI) and conjugated linoleic acid (CLA) on mammary and hepatic lipogenesis in lactating C57Bl/6 J mice. Twenty‐four lactating mice were randomly assigned to one of four treatments applied from postpartum day 6 to day 10. Treatments included: (1) control diet, (2) control plus 1.5 % dietary CLA (CLA) substituted for soybean oil, (3) control plus daily intra‐peritoneal (IP) rosiglitazone injections (10 mg/kg body weight) (ROSI), and (4) CLA plus ROSI (CLA‐ROSI). Dam food intake and milk fat concentration were depressed with CLA. However, no effects were observed with ROSI. The CLA‐induced milk fat depression was due to reduced expression for mammary lipogenic genes involved in de‐novo fatty acid (FA) synthesis, FA uptake and desaturation, and triacyglycerol synthesis. Liver weight (g/100 g body weight) was increased by CLA due to an increase in lipid accumulation triggering a compensatory reduction in mRNA abundance of hepatic lipogenic enzymes, including acetyl‐CoA carboxylase I and stearoyl‐CoA desaturase I. On the contrary, no effects were observed with ROSI on hepatic and mammary lipogenic gene and enzyme expression. Overall, feeding CLA to lactating mice induced milk fat depression and increased hepatic lipid accumulation, probably due to the presence of trans ‐10, cis ‐12 CLA isomer, while ROSI failed to significantly attenuate both hepatic steatosis and reduction in milk fat content.