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Triolein and Trilinolein Ameliorate Oxidized Low‐Density Lipoprotein‐Induced Oxidative Stress in Endothelial Cells
Author(s) -
Luo Ting,
Deng Zeyuan,
Li Xiaoping,
Rao Huan,
Fan Yawei
Publication year - 2014
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-014-3889-4
Subject(s) - triolein , chemistry , oxidative stress , lipoprotein , antioxidant , viability assay , apoptosis , biochemistry , high density lipoprotein , low density lipoprotein , endothelial stem cell , cholesterol , in vitro , enzyme , lipase
Abstract Uptake of oxidized low‐density lipoprotein by endothelial cells is a critical step for the initiation of atherosclerosis. Triacylglycerol uptake in these cells is understood to be a part of the process. The present investigation, comparison among the effects of simple acylglycerol, including tristearin, triolein, and trilinolein, upon oxidized low‐density lipoprotein ‐induced oxidative stress was undertaken. Results indicated that trilinolein (78 % ± 0.02) and triolein (90 % ± 0.01) increased cell viability of endothelial cells exposed to oxidized low‐density lipoprotein, whereas tristearin decreased the cell viability (55 % ± 0.03) ( P < 0.05). Oxidized low‐density lipoprotein treatment significantly increased apoptosis (23 %), compared to cells simultaneously exposed to trilinolein (19 %) or triolein (16 %), where apoptosis was reduced ( P < 0.05). On the other hand, exposure to tristearin further increased oxidized low‐density lipoprotein ‐induced cell apoptosis (34 %). Treatment with trilinolein or triolein on oxidized low‐density lipoprotein ‐stimulated endothelial cells inhibited the expression of ICAM‐1 and E‐selectin mRNA. Moreover, both trilinolein and triolein demonstrated a strong antioxidant response to oxidative stress caused by oxidized low‐density lipoprotein. Taken together, the results indicate trilinolein and triolein possess anti‐inflammatory properties, which are mediated via the antioxidant defense system.