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Identification of Plasmalogen Cardiolipins from Pectinatus by Liquid Chromatography–High Resolution Electrospray Ionization Tandem Mass Spectrometry
Author(s) -
Řezanka Tomáš,
Matoulková Dagmar,
Kyselová Lucie,
Sigler Karel
Publication year - 2013
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-013-3851-x
Subject(s) - plasmalogen , chemistry , cardiolipins , chromatography , electrospray ionization , tandem mass spectrometry , mass spectrometry , orbitrap , electrospray , resolution (logic) , tripalmitin , biochemistry , phospholipid , cardiolipin , artificial intelligence , membrane , computer science
High resolution electrospray ionization tandem mass spectrometry (HR‐ESI–MS/MS) was used to analyze cardiolipins (Ptd 2 Gro) including their plasmalogen forms from three species of the anaerobic beer‐spoilage bacterial genus Pectinatus . Cardiolipins including their plasmalogens were analyzed by HR‐ESI–MS/MS on Orbitrap and almost 100 cardiolipins (i.e. tetra‐acyl—Ptd 2 Gro, plasmenyl‐tri‐acyl—PlsPtd 2 Gro, and di‐plasmenyl‐di‐acyl—Pls 2 Ptd 2 Gro) of three classes were identified. The structures of the molecular species that consist of various regioisomers and structurally similar compounds were revealed in detail. The high resolution mass spectrometry allowed the unambiguous structural assignment of Ptd 2 Gro, PlsPtd 2 Gro, and Pls 2 Ptd 2 Gro in the three species of Pectinatus , which contain predominantly odd numbered fatty acids.