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The β‐Subunit of Cholera Toxin has a High Affinity for Ganglioside GM1 Embedded into Solid Supported Lipid Membranes with a Lipid Raft‐Like Composition
Author(s) -
Margheri G.,
D'Agostino R.,
Trigari S.,
Sottini S.,
Del Rosso M.
Publication year - 2014
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-013-3845-8
Subject(s) - cholera toxin , ganglioside , lipid raft , sphingomyelin , membrane , raft , chemistry , lipid bilayer , biophysics , biochemistry , liposome , microbiology and biotechnology , biology , organic chemistry , copolymer , polymer
In this communication, we report on the fabrication of GM1‐rich solid‐supported bilayer lipid membranes (ssBLM) made of sphingomyelin and cholesterol, the main components of lipid rafts, which are the physiological hosting microenvironment of GM1 on the cell membrane. The functionality of the ganglioside has been checked by measuring the apparent dissociation constant K D of the complex formed by the β‐subunit of the cholera toxin and GM1. The value found deviates less than one order of magnitude from that measured for in vivo cells, indicating the potential of these ssBLM as optimized in vitro biomimetic platforms.