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Oral Administration of Oleic or Linoleic Acids Modulates the Production of Inflammatory Mediators by Rat Macrophages
Author(s) -
Magdalon Juliana,
Vinolo Marco A. R.,
Rodrigues Hosana G.,
Paschoal Vivian A.,
Torres Rosângela P.,
ManciniFilho Jorge,
Calder Philip C.,
Hatanaka Elaine,
Curi Rui
Publication year - 2012
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-012-3687-9
Subject(s) - lipidology , lipopolysaccharide , clinical chemistry , chemistry , superoxide , linoleic acid , oleic acid , oral administration , biochemistry , macrophage , pharmacology , endocrinology , medicine , fatty acid , enzyme , in vitro
Oleic (OLA) and linoleic (LNA) acids are commonly consumed fatty acids and they can modulate the inflammatory response, in which macrophages play an important role. The aim of this study was to investigate the effects of these two fatty acids on the production of inflammatory mediators by macrophages. Rats received oral administration of water (control), OLA or LNA (0.22 g/kg body weight) daily for 10 days and peritoneal resident macrophages were then isolated. Subsequently, they were seeded in culture plates and the production of various inflammatory mediators was assessed. Oral administration with OLA decreased the production of IL‐1β, IL‐6 and CINC‐2αβ by resident macrophages and LNA decreased the production of IL‐1β, IL‐6 and VEGF in the absence of lipopolysaccharide (LPS), although it accelerated IL‐1β release and decreased IL‐10 synthesis when cells were stimulated with LPS. Neither fatty acid affected the production of superoxide anion, hydrogen peroxide, nitrite, TNF‐α, PGE 2 , LTB 4 or 15( S )‐HETE. Thus, OLA and LNA influence the production of several inflammatory mediators by macrophages.