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The Composition of the Cuticular and Internal Free Fatty Acids and Alcohols from Lucilia sericata Males and Females
Author(s) -
Gołębiowski Marek,
Boguś Mieczysława I.,
Paszkiewicz Monika,
Wieloch Wioletta,
Włóka Emilia,
Stepnowski Piotr
Publication year - 2012
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-012-3662-5
Subject(s) - lucilia , lipidology , biology , cholesterol , clinical chemistry , sterol , tephritidae , biochemistry , chemistry , botany , calliphoridae , pest analysis , larva
GC, GC–MS, and HPLC–LLSD analyses were used to identify and quantify cuticular and internal lipids in males and females of the blow‐fly ( Lucilia sericata ). Sixteen free fatty acids, seven alcohols and cholesterol were identified and quantitatively determined in the cuticular lipids of L. sericata . Cuticular fatty acids ranged from C 6 to C 20 and included unsaturated entities such as 16:1n‐9, 18:1n‐9, 20:4n‐3 and 20:5n‐3. Cuticular alcohols (only saturated and even‐numbered) ranged from C 12 to C 20 in males and C 10 to C 22 in females. Only one sterol was found in the cuticular lipids of both males and females. 23 free fatty acids, five alcohols and cholesterol were identified in the internal lipids. Internal fatty acids were present in large amounts—7.4 mg/g (female) and 10.1 mg/g (male). Only traces of internal alcohols (from C 14 to C 26 in males, from C 14 to C 22 in females) were found in L. sericata . Large amounts of internal cholesterol were identified in L. sericata males and females (0.49 and 0.97 mg/g of the insect body, respectively).