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Separation of Lipid Classes by HPLC on a Cyanopropyl Column
Author(s) -
Olsson Petter,
Holmbäck Jan,
Herslöf Bengt
Publication year - 2012
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-011-3627-0
Subject(s) - lipidology , chromatography , column (typography) , separation (statistics) , chemistry , high performance liquid chromatography , clinical chemistry , separation method , analytical chemistry (journal) , mathematics , biochemistry , statistics , geometry , connection (principal bundle)
A new method for the separation and identification of lipid classes by normal‐phase HPLC on a cyanopropyl column is described. The use of a simple binary gradient, with toluene as a component, provided a rapid separation of non‐polar as well as phospholipid classes. The inherent small differences in performances between possible non‐polar eluent components of the gradient, such as hexane, heptane, and iso‐octane, had a pronounced impact on retention times for individual phospholipid classes. Separation of molecular species within a lipid class could also be observed.