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2‐Polyunsaturated Acyl Lysophosphatidylethanolamine Attenuates Inflammatory Response in Zymosan A‐Induced Peritonitis in Mice
Author(s) -
Hung Nguyen Dang,
Kim Mee Ree,
Sok DaiEun
Publication year - 2011
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-011-3589-2
Subject(s) - zymosan , chemistry , lysophosphatidylethanolamine , polyunsaturated fatty acid , pharmacology , peritonitis , clinical chemistry , extravasation , lipoxin , biochemistry , inflammation , endocrinology , medicine , immunology , phospholipid , phosphatidylcholine , in vitro , fatty acid , membrane , receptor
In the present study, the anti‐inflammatory action of lysophosphatidylethanolamine (lysoPtdEtn), orally administered, in zymosan A‐induced peritonitis was examined. Oral administration of 2‐DHA‐lysoPtdEtn (ED 50 , ~111 μg/kg) or 2‐ARA‐lysoPtdEtn (ED 50 , 221 μg/kg) was found to inhibit the plasma leakage in mice treated with zymosan A. In support of this, 2‐polyunsaturated acyl‐lysoPtdEtn diminished the formation of LTC 4 , a lipid mediator responsible for vascular permeability. Next, 2‐DHA‐lysoPtdEtn (ED 50 , 110 μg/kg) or 2‐ARA‐lysoPtdEtn (ED 50 , 123 μg/kg) effectively inhibited the leukocyte extravasation into the peritoneum. Consistent with this, each polyunsaturated‐lysoPtdEtn diminished the formation of LTB 4 and 12‐HETE, potent chemotactic factors. Additionally, the level of pro‐inflammatory mediator (IL‐1 β, IL‐6, TNF‐α or NO) was lowered remarkably in contrast to the augmentation of anti‐inflammatory interleukin IL‐10. Furthermore, 2‐(15‐HETE)‐lysoPtdEtn and 2‐(17‐HDHE)‐lysoPtdEtn, 15‐lipoxygenation product of 2‐ARA‐lysoPtdEtn and 2‐DHA‐lysoPtdEtn, respectively, were more potent than corresponding lysoPtdEtn, suggesting the action of 2‐acyl‐lysoPtdEtn might be expressed through 15‐lipoxygenation. In support of this, the formation of 15‐HETE and LXA 4 was upgraded in accordance with an increasing dose of 2‐ARA‐lysoPtdEtn. Separately, anti‐inflammatory actions, 2‐polyunsaturated acyl‐lysoPtdEtns also drastically diminished leukocyte infiltration in a later phase of zymosan A‐induced peritonitis, indicating that these lipids also possess pro‐resolving activity. Taken together, it is suggested that polyunsaturated lysoPtdEtns and their lipoxygenation derivatives, could be classified as potent anti‐inflammatory lipids.