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Amadori‐Glycated Phosphatidylethanolamine, a Potential Marker for Hyperglycemia, in Streptozotocin‐Induced Diabetic Rats
Author(s) -
Sookwong Phumon,
Nakagawa Kiyotaka,
Fujita Ikuko,
Shoji Naoki,
Miyazawa Teruo
Publication year - 2011
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-011-3588-3
Subject(s) - amadori rearrangement , glycation , diabetes mellitus , medicine , streptozotocin , endocrinology , phosphatidylethanolamine , chemistry , biochemistry , phospholipid , phosphatidylcholine , membrane
It has been demonstrated in vivo that lipid glycation products such as Amadori‐glycated phosphatidylethanolamine (Amadori‐PE) accumulate in the plasma of diabetic humans and animals, but how lipid glycation products are formed under hyperglycemic conditions are not clear. We sought to clarify the occurrence of lipid glycation and its relationships with lipid peroxidation and protein glycation during the development of hyperglycemia using the streptozotocin (STZ)‐induced diabetic rat model. A significant increase in Amadori‐PE was observed in STZ rats 7 days after STZ treatment, and Amadori‐PE (especially 18:0–20:4 Amadori‐PE) was found at high levels in the blood and in organs that are strongly affected by diabetes, such as the kidney. Significant changes in Amadori‐PE appeared to occur prior to changes in levels of oxidized lipids, which increased after 21–28 days. In addition, accumulation of N ε‐(carboxymethyl)lysine (CML), a protein glycation product, proceeded somewhat more slowly and moderately than that of Amadori‐PE, suggesting that Amadori‐PE and CML are early and advanced glycation products, respectively. Our results suggest that Amadori‐PE may be a useful predictive marker for hyperglycemia, particularly in the early stages of diabetes. Similar speculations have been made from previous human studies, but this study provides a direct evidence to support the speculations in rat study.