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Stratum Corneum Lipid Structure Investigated by EPR Spin‐Probe Method: Application of Terpenes
Author(s) -
Nakagawa Kouichi,
Anzai Kazunori
Publication year - 2010
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-010-3479-z
Subject(s) - stratum corneum , lipidology , terpene , electron paramagnetic resonance , clinical chemistry , chemistry , corneocyte , organic chemistry , nuclear magnetic resonance , biology , biochemistry , physics , genetics
Electron paramagnetic resonance (EPR) in conjunction with a slow‐tumbling simulation was utilized for defining stratum corneum (SC) lipid structure. SC from the back of hairless mouse (HOS:HR‐1) was stripped consecutively from one to three or four times using a glass plate coated with a cyanoacrylate resin. Aliphatic spin probes, 5‐doxylstearic acid (5‐DSA) and 3β‐doxyl‐5α‐cholestane (CHL), were used to evaluate the SC ordering. EPR spectrum of 5‐DSA incorporated in the SC demonstrated a characteristic peak for the first strip. A slow‐tumbling simulation for 5‐DSA showed clear differences in EPR intensities as well as ordering values ( S 0 ) of the SC for control and terpenes treated SC. The α‐terpineol enhanced the permeation of the single chain 5‐DSA about three times more than that of the control. However, EPR spectra of CHL in the SC did not show a clear difference for each strip, except for the signal intensity. The results imply that CHL permeates into SC lipid differently from 5‐DSA. The enhancement of the 5‐DSA is more significant than that of CHL. Therefore, the present results can be useful for various drug administrations via the skin.