Premium
Purified Canola Lutein Selectively Inhibits Specific Isoforms of Mammalian DNA Polymerases and Reduces Inflammatory Response
Author(s) -
Horie Sho,
Okuda Chiaki,
Yamashita Takatoshi,
Watanabe Kenichi,
Kuramochi Kouji,
Hosokawa Masashi,
Takeuchi Toshifumi,
Kakuda Makiko,
Miyashita Kazuo,
Sugawara Fumio,
Yoshida Hiromi,
Mizushina Yoshiyuki
Publication year - 2010
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-010-3449-5
Subject(s) - lutein , carotenoid , zeaxanthin , xanthophyll , canola , astaxanthin , biochemistry , biology , rapeseed , antioxidant , food science
In the screening of DNA polymerase (pol) inhibitor, we isolated lutein, a carotenoid, from the crude (unrefined) pressed oil of canola (low erucic acid rapeseed, Brassica napus L.). Commercially prepared carotenoids such as lutein ( 1 ), zeaxanthin ( 2 ), β‐cryptoxanthin ( 3 ), astaxanthin ( 4 ), canthaxanthin ( 5 ), β‐carotene ( 6 ), lycopene ( 7 ), capsanthin ( 8 ), fucoxanthin ( 9 ) and fucoxanthinol ( 10 ), were investigated for the inhibitory activities of pols. Compounds 1 , 2 and 8 exhibited strong inhibition of the activities of mammalian pols β and λ, which are DNA repair‐ and/or recombination‐related pols. On the other hand, all carotenoids tested had no influence on the activity of a mammalian pol α, which is a DNA replicative pol. Lutein ( 1 ) was the strongest pol inhibitor of mammalian pols β and λ in the prepared ten carotenoids tested, but did not influence of the activities of mammalian pols α, γ, δ and ε. The tendency for pols β and λ inhibition by these carotenoids showed a positive correlation with the suppression of TPA (12‐ O ‐tetradecanoylphorbol‐13‐acetate)‐induced inflammation. These results suggest that cold pressed unrefined canola/rapeseed oil, or other oils with high levels of lutein and other carotenoids, may be useful for their anti‐inflammatory properties.