Premium
Trans Fatty Acid‐Induced NF‐κB Activation Does Not Induce Insulin Resistance in Cultured Murine Skeletal Muscle Cells
Author(s) -
Hommelberg Pascal P. H.,
Langen Ramon C. J.,
Schols Annemie M. W. J.,
Essen A L. M.,
Snepvangers Frank J. M.,
Mensink Ronald P.,
Plat Jogchum
Publication year - 2010
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-010-3388-1
Subject(s) - insulin resistance , skeletal muscle , glut4 , medicine , elaidic acid , endocrinology , insulin , transactivation , fatty acid , palmitic acid , chemistry , lipotoxicity , saturated fatty acid , biology , biochemistry , linoleic acid , transcription factor , gene
Long‐chain saturated fatty acids such as palmitic acid induce insulin resistance and NF‐κB activation in skeletal muscle cells. Here we investigated the effects of long‐chain fatty acid (FA) saturation and configuration on NF‐κB activity and insulin sensitivity in cultured skeletal muscle cells. Of all tested unsaturated FAs, only elaidic acid (3‐fold), cis 9, trans 11‐CLA (3‐fold) and trans 10, cis 12‐CLA (13‐fold) increased NF‐κB transactivation in myotubes. This was not accompanied by decreased insulin sensitivity (measured as insulin‐induced glucose uptake and GLUT4 translocation). We therefore conclude that FA‐induced NF‐κB activation is not sufficient for the induction of insulin resistance in skeletal muscle cells.