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Chemical Structure of Bacteriovorax stolpii Lipid A
Author(s) -
Beck Sebastian,
Müller Frederic D.,
Strauch Eckhard,
Brecker Lothar,
Linscheid Michael W.
Publication year - 2010
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-010-3383-6
Subject(s) - chemistry , mass spectrometry , electrospray ionization , lipid a , chromatography , nuclear magnetic resonance spectroscopy , lipidology , fatty acid , chemical structure , polyacrylamide gel electrophoresis , biochemistry , stereochemistry , organic chemistry , bacteria , biology , enzyme , genetics
Bdellovibrionales is a phylogenetically diverse group of predatory prokaryotes, which consists of the two families Bdellovibrionaceae and Bacteriovoracaceae . We describe LPS and lipid A of the type strain Bacteriovorax stolpii DSM 12778, representing the first characterized endotoxin of a Bacteriovoracaceae member. It has a smooth form LPS, which was identified by SDS‐polyacrylamide gel electrophoresis. The lipid A structure was determined by combined gas chromatography–mass spectrometry, electrospray ionization mass spectrometry and NMR spectroscopy. Its backbone consists of two β‐(1 → 6)‐linked 2,3‐diamino‐2,3‐dideoxy‐ d ‐glucopyranoses (Glc p N3N) carrying a pyrophosphoethanolamine at O‐4′ of the non‐reducing sugar and a phosphate group linked to O‐1 of the reducing Glc p N3N. Positions 2, 3, 2′ and 3′ of the two Glc p N3N are acylated with primary 3‐hydroxy fatty acids and one of those carries a secondary fatty acid.