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Effect of trans 8, cis 10+ cis 9, trans 11 Conjugated Linoleic Acid Mixture on Lipid Metabolism in 3T3‐L1 Cells
Author(s) -
Joseph Shama V.,
Miller Jessica R.,
McLeod Roger S.,
Jacques Hélène
Publication year - 2009
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-009-3309-3
Subject(s) - conjugated linoleic acid , triglyceride , lipidology , chemistry , linoleic acid , clinical chemistry , metabolism , adiponectin , fatty acid , biochemistry , endocrinology , cholesterol , biology , obesity , insulin resistance
Evidence suggests that minor isomers of conjugated linoleic acid (CLA), such as trans 8, cis 10 CLA, can elicit unique biological effects of their own. In order to determine the effect of a mixture of t 8, c 10+ c 9, t 11 CLA isomers on selected aspects of lipid metabolism, 3T3‐L1 preadipocytes were differentiated for 8 days in the presence of 100 μM linoleic acid (LA); t 8, c 10+ c 9, t 11 CLA; t 10, c 12+ c 9, t 11 CLA or purified c 9, t 11 CLA. Whereas supplementation with c 9, t 11 and t 10, c 12+ c 9, t 11 CLA resulted in cellular triglyceride (TG) concentrations of 3.4 ± 0.26 and 1.3 ± 0.11 μg TG/μg protein, respectively ( P < 0.05), TG accumulation following treatment with CLA mixture t 8, c 10+ c 9, t 11 was significantly intermediate (2.5 ± 0.22 μg TG/μg protein, P < 0.05) between the two other CLA treatments. However, these effects were not attributable to an alteration of the Δ 9 desaturation index. Adiponectin content of adipocytes treated with t 8, c 10+ c 9, t 11 mixture was similar to the individual isomer c 9, t 11 CLA, and both the t 8, c 10+ c 9, t 11 and c 9, t 11 CLA groups were greater ( P < 0.05) than in the t 10, c 12+ c 9, t 11 CLA group. Overall, these results suggest that t 8, c 10+ c 9, t 11 CLA mixture affects TG accumulation in 3T3‐L1 cells differently from the c 9, t 11 and t 10, c 12 isomers. Furthermore, the reductions in TG accumulation occur without adversely affecting the adiponectin content of these cells.