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Over‐expression of the Anti‐apoptotic Protein Bcl‐2 Affects Membrane Lipid Composition in HL‐60 Cells
Author(s) -
Cantrel Catherine,
Zachowski Alain,
Geny Blandine
Publication year - 2009
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-009-3292-8
Subject(s) - chemistry , fatty acid , clinical chemistry , apoptosis , biochemistry , mitochondrion , reactive oxygen species , lipidology , unsaturated fatty acid , membrane , oxidative phosphorylation , plasmalogen , eukaryotic cell , cell , phospholipid
We studied modifications induced at the membrane lipid level by over‐expression of the anti‐apoptotic protein Bcl‐2. When total cell phospholipids were analyzed, the transformation led to a moderate decrease in poly‐unsaturated fatty acids, compensated by an increase in mono‐unsaturated species. At the mitochondrial membrane level, the changes were more important and occurred in saturated and dimethyl acetal fatty acids, which became more abundant, while unsaturated fatty acid content diminished. This indicates a decline in oxidation‐sensitive fatty acids (unsaturated species) together with a gain in oxidation‐insensitive saturated fatty acids and in plasmalogen (as detected by dimethyl acetal fatty acids) considered as oxygen species scavengers. Theses changes, combined with the protective role of Bcl‐2 against oxidation due to its effect on the redox potential, should protect cells from apoptosis starting in mitochondria.