Premium
Inhibitory Effect of Conjugated α‐Linolenic Acid from Bifidobacteria of Intestinal Origin on SW480 Cancer Cells
Author(s) -
Coakley Mairéad,
Banni Sebastiano,
Johnson Mark C.,
Mills Susan,
Devery Rosaleen,
Fitzgerald Gerald,
Paul Ross R.,
Stanton Catherine
Publication year - 2009
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-008-3269-z
Subject(s) - lipidology , clinical chemistry , conjugated system , chemistry , inhibitory postsynaptic potential , linolenic acid , food science , biochemistry , biology , fatty acid , organic chemistry , endocrinology , linoleic acid , polymer
In this study, we assessed the ability of six strains of bifidobacteria (previously shown by us to possess the ability to convert linoleic acid to c 9, t 11‐conjugated linoleic acid (CLA) to grow in the presence of α‐linolenic acid and to generate conjugated isomers of the fatty acid substrate during fermentation for 42 h. The six strains of bifidobacteria were grown in modified MRS (mMRS) containing α‐linolenic acid for 42 h at 37 °C, after which the fatty acid composition of the growth medium was assessed by gas liquid chromatography (GLC). Indeed, following fermentation of one of the strains, namely Bifidobacterium breve NCIMB 702258, in the presence of 0.41 mg/ml α‐linolenic acid, 79.1% was converted to the conjugated isomer, C18:3 c 9, t 11, c 15 conjugated α‐linolenic acid (CALA). To examine the inhibitory effect of the fermented oils produced, SW480 colon cancer cells were cultured in the presence of the extracted fermented oil (10–50 μg/ml) for 5 days. The data indicate an inhibitory effect on cell growth ( p ≤ 0.001) of CALA, with cell numbers reduced by 85% at a concentration of 180 μM, compared with a reduction of only 50% with α‐linolenic acid ( p ≤ 0.01).