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Effect of a Seaweed Extract on Fatty Acid Accumulation and Glycerol‐3‐Phosphate Dehydrogenase Activity in 3T3‐L1 Adipocytes
Author(s) -
He M. L.,
Wang Y.,
You J. S.,
Mir P. S.,
McAllister T. A.
Publication year - 2009
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-008-3256-4
Subject(s) - oleic acid , fatty acid , dehydrogenase , palmitoleic acid , chemistry , biochemistry , fatty acid synthesis , glycerol , adipocyte , linoleic acid , adipose tissue , enzyme
This study was to determine the effect of a seaweed Ascophyllum nodosum extract (SE) containing 220 mg g −1 phlorotannins on differentiation and fatty acid accumulation in differentiating 3T3‐L1 adipocytes. 3T3‐L1 cells (2 × 10 4 mL −1 ) were seeded to 24‐well plates and proliferated to reach confluence and then were treated with media containing 0, 12.5, 25, 50, 75 and 100 μg mL −1 SE for 8 days. Dexamethasone, methyl‐isobutylxanthine and insulin (DMI) were added to the media in the first 2 days to induce cell differentiation. On day 8 the adipocytes were harvested for measuring cellular fatty acid concentration and the activity of glycerol‐3‐phosphate dehydrogenase (GPDH). It was found that treatment with SE increased ( P < 0.01, n = 6) cellular myristoleic acid (C14:1), palmitoleic acid (C16:1) and oleic acid (C18:1) and total monounsaturated fatty acids (MUFA) without significantly affecting the cell number and saturated fatty acid (SFA). Ratios of MUFA/SFA, C14:1/C14:0, C16:1/C16:0 and C18:1/C18:0 in cellular lipids increased ( P < 0.05, n = 6) with the SE treatment in a dose dependent manner ( P < 0.001). Treatment with 75 μg mL −1 SE depressed ( P < 0.05) cellular GPDH activity. The results indicate that the biological factors in the SE may be involved in differentiation and MUFA accumulation in adipocytes.