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Determination of Triacylglycerols in Butterfat by Normal‐Phase HPLC and Electrospray–Tandem Mass Spectrometry
Author(s) -
Kalo P.,
Kemppinen A.,
Ollilainen V.
Publication year - 2009
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-008-3247-5
Subject(s) - chemistry , butterfat , chromatography , mass spectrometry , electrospray ionization , tandem mass spectrometry , composition (language) , analytical chemistry (journal) , organic chemistry , milk fat , linseed oil , linguistics , philosophy
Here, we report the identification and quantification of the molecular species of long‐, medium‐, and short‐chain triacylglycerols (TAG) in butterfat (BF), including TAG with an odd number of acyl carbons (ACN) and TAG with unidentified molecular species. In the present study, in addition to auto‐MS 2 , a large number of methods, each recording MS 2 for 1–4 ions, were used for identification of TAG species. For the quantification of long‐chain, odd ACN TAG, and TAG with unidentified molecular species, molar correction factors (MCF) were calculated from the uncorrected mol% (area mol%) of each ACN:DB (number of double bonds) class in randomized butterfat (RBF), and the respective mol% in the calculated random composition of RBF. The butyrate, caproate, and medium‐chain (C 8 , C 10 ) TAG were quantified using regio‐ or acyl‐chain‐specific MCF calculated from their area mol% in RBF and mol% in the calculated random composition. These methods enabled us to identify ca. 450 TAG species in 184 quantified peaks of 88 ACN:DB classes. The proportions of saturated, monoene, diene, triene, tetraene, pentaene, and hexaene TAG were 40.0, 38.4, 16.2, 4.5, 0.6, 0.1, and 0.03 mol%, respectively. The proportions of TAG with not identified molecular species and odd ACN TAG were 11.8 and 5.7 mol%, respectively. The most abundant short‐chain TAG species were butyroyldipalmitoylglycerol + butyroylmyristoylstearoylglycerol (5.25 mol%) and butyroylpalmitoyloleoylglycerol (4.08 mol%).