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Dietary Perilla Oil Inhibits Proinflammatory Cytokine Production in the Bronchoalveolar Lavage Fluid of Ovalbumin‐Challenged Mice
Author(s) -
Chang HuiHsiang,
Chen ChinShun,
Lin JinYuarn
Publication year - 2008
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-008-3171-8
Subject(s) - proinflammatory cytokine , bronchoalveolar lavage , perilla , polyunsaturated fatty acid , cytokine , corn oil , immunology , ovalbumin , inflammation , chemistry , pharmacology , medicine , endocrinology , food science , biochemistry , fatty acid , immune system , lung , raw material , organic chemistry
To evaluate the anti‐inflammatory effects of different dietary oils on ovalbumin‐sensitized and ‐challenged mice. Experimental BALB/c mice were fed with different diets containing 5% corn oil [rich in linoleic acid, 18:2n‐6 polyunsaturated fatty acids (PUFA), as a control diet], 5% perilla oil (rich in α‐linolenic acid, 18:3n‐3 PUFA) or 5% compound oil containing 50% corn oil and 50% perilla oil, for 5 consecutive weeks. The leukocyte count, inflammatory mediators, and cytokine levels, including proinflammatory and Th1/Th2 cytokines in the bronchoalveolar lavage fluid (BALF) from the mice were determined. The results showed that 5% compound oil administration significantly ( P < 0.05) decreased eosinophilic infiltration. Dietary perilla oil could not significantly ( P > 0.05) decrease the eosinophil accumulation or the secretions of inflammatory mediators such as prostaglandin E 2 (PGE 2 ), histamine, nitric oxide and eotaxin. However, dietary perilla oil significantly ( P < 0.05) reduced proinflammatory cytokine (TNF‐α, IL‐1β and IL‐6) and Th1 cytokine (IFN‐γ and IL‐2) production. The production of Th2 cytokine IL‐10, but not IL‐4 and IL‐5, was also significantly inhibited by perilla oil administration. The results suggest that dietary perilla oil might alleviate inflammation via decreasing the secretion of pro‐inflammatory cytokines in BALF, but failed to regulate the Th1/Th2 balance toward Th1 pole during the Th2‐skewed allergic airway inflammation.