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The Effects of Omega‐3 Polyunsaturated Fatty Acids on TNF‐α and IL‐10 Secretion by Murine Peritoneal Cells In Vitro
Author(s) -
Skuladottir Ingibjorg H.,
Petursdottir Dagbjort H.,
Hardardottir Ingibjorg
Publication year - 2007
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/s11745-007-3081-1
Subject(s) - docosahexaenoic acid , polyunsaturated fatty acid , eicosapentaenoic acid , arachidonic acid , tumor necrosis factor alpha , biology , secretion , interleukin 10 , cytokine , endocrinology , immune system , medicine , fatty acid , immunology , biochemistry , enzyme
Omega‐3 polyunsaturated fatty acids (PUFA) affect immune response, partly by affecting cytokine secretion. Omega‐3 PUFA decrease tumor necrosis factor (TNF)‐α secretion by RAW 264.7 macrophages but increase TNF‐α secretion by primary elicited peritoneal macrophages in vitro. In this study, the effects of omega‐3 and omega‐6 PUFA on lipopolysaccharide induced TNF‐α and interleukin (IL)‐10 secretion by murine primary resident and elicited peritoneal macrophages and by RAW 264.7 macrophages, were examined in vitro using an enzyme‐linked immunosorbent assay. In addition, the effects of dietary omega‐3 PUFA on the number of cells secreting these cytokines were examined with enzyme‐linked immunospot assay. All cell types secreted more TNF‐α but similar amounts of IL‐10 when incubated with the omega‐3 PUFA, eicosapentaenoic acid or docosahexaenoic acid, compared with that when incubated with the omega‐6 PUFA, linoleic acid or arachidonic acid. Dietary fish oil did not affect the number of TNF‐α secreting resident peritoneal macrophages but decreased the number of macrophages secreting IL‐10 ex vivo. These results show that dietary omega‐3 PUFA and omega‐3 PUFA added to cells in vitro increase TNF‐α secretion by resident peritoneal macrophages, probably by a direct effect on the cells. In contrast, omega‐3 PUFA did not affect IL‐10 secretion by the cells but decreased the number of cells secreting IL‐10 ex vivo, possibly by affecting cell recruitment, maturation or proliferation.